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Masson’s Trichrome for Connective Tissue

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Masson’s trichrome is a three-colour staining protocol used in histology. The recipes evolved from Claude L. Pierre Masson‘s (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue.

Sheehan and Hrapchak (1980)

For stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc.

Technical Data Sheet

Description

Stain results:
Nuclei: Black
Cytoplasm, Keratin, Muscle Fibers: Red
Collagen, Mucin: Blue
MSDS 26367-01MSDS 26367-02MSDS 26367-03MSDS 26367-04
MSDS 26367-05MSDS 26367-06MSDS 26367-07 

References:
Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190F.3 Elastic Tissue Stains