{"id":20781,"date":"2025-09-08T15:44:53","date_gmt":"2025-09-08T13:44:53","guid":{"rendered":"https:\/\/delta.screenup.com\/produit\/mops-biological-buffer\/"},"modified":"2025-09-08T15:44:58","modified_gmt":"2025-09-08T13:44:58","slug":"mops-biological-buffer","status":"publish","type":"product","link":"https:\/\/delta.screenup.com\/en\/produit\/mops-biological-buffer\/","title":{"rendered":"Mops, Biological Buffer"},"content":{"rendered":"<pre class=\"sp_pageHeader\"><strong><span style=\"font-size: 14pt;\">MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe<\/span><\/strong><\/pre>\n<p>T<strong>o prepare\u00a0 1 liter of MOPS Buffe<\/strong>r (10X) (0.2 M, pH 7):<\/p>\n<div>\n<div id=\"bufferContainer\">\n<div>Table 1. Required components<\/div>\n<div id=\"bufferOutput\">\n<table id=\"componentsTable\">\n<tbody>\n<tr>\n<td><strong>Component<\/strong><\/td>\n<td><strong>Mass<\/strong><\/td>\n<td><strong>Molarity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>MOPS free acid (mw: 209.26 g\/mol)<\/td>\n<td>41.86 g<\/td>\n<td>0.2 M<\/td>\n<\/tr>\n<tr>\n<td>Sodium Acetate (mw: 82.03 g\/mol)<\/td>\n<td>4.1 g<\/td>\n<td>0.05 M<\/td>\n<\/tr>\n<tr>\n<td>Na<sub>2<\/sub>EDTA (mw: 372.24 g\/mol)<\/td>\n<td>3.72 g<\/td>\n<td>0.01 M<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ol id=\"compInst\">\n<li>Prepare 800 mL of dH2O in a suitable container.<\/li>\n<li>Add 41.86 g of MOPS free acid to the solution.<\/li>\n<li>Add 4.1 g of Sodium Acetate to the solution.<\/li>\n<li>Add 3.72 g of Na<sub>2<\/sub>EDTA to the solution.<\/li>\n<li>Adjust solution to desired pH using NaOH (typical pH = 7)<\/li>\n<li>Add distilled water until volume is 1 L.<\/li>\n<\/ol>\n<\/div>\n<\/div>\n<\/div>\n<p>\u00a0<\/p>\n<p><strong>storage : 0-5\u00b0C<\/strong><\/p>\n<p><span style=\"font-size: 14pt;\"><a href=\"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2019\/03\/MSDS_18900.pdf\">MSDS_18900<\/a> \u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>(3-(N-Morpholino) propanesulfonic acid) pH range 6.5-7.9 \u00a0<\/p>\n<p><strong><span style=\"font-size: 12pt;\">unit. 100 gr<\/span><\/strong><\/p>\n<p><b>MOPS<\/b>\u00a0(3-(N-morpholino)propanesulfonic acid) is a\u00a0<b>buffer<\/b>\u00a0introduced by Good et al. &#8230;<\/p>\n<p>Its chemical structure contains a morpholine ring. HEPES is a similar pH\u00a0<b>buffering<\/b>\u00a0compound that contains a piperazine ring. With a pK<sub>a<\/sub>\u00a0of 7.20,\u00a0<\/p>\n<p><b>MOPS<\/b>\u00a0is an excellent\u00a0<b>buffer<\/b>\u00a0for many\u00a0<b>biological<\/b>\u00a0systems at near-neutral pH.<\/p>\n","protected":false},"featured_media":20782,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_nectar_blocks_hide_post_title":false,"_nectar_blocks_transparent_header_effect":false,"_nectar_blocks_transparent_header_effect_color":"light","_nectar_blocks_header_animation":false,"_nectar_blocks_header_animation_delay":0,"_nectar_blocks_header_animation_effect":"fade","_nectar_blocks_page_css":"","_nectar_blocks_page_js":""},"product_brand":[],"product_cat":[1354,1349,1350,1353],"product_tag":[],"class_list":["post-20781","product","type-product","status-publish","has-post-thumbnail","product_cat-autres-tampons","product_cat-met","product_cat-reactifs","product_cat-tampons","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"_links":{"self":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product\/20781","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media\/20782"}],"wp:attachment":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media?parent=20781"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_brand?post=20781"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_cat?post=20781"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_tag?post=20781"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}