{"id":21260,"date":"2025-09-08T15:53:45","date_gmt":"2025-09-08T13:53:45","guid":{"rendered":"https:\/\/delta.screenup.com\/produit\/formalin-buffered-10-in-phosphate-buffer\/"},"modified":"2025-09-09T12:48:14","modified_gmt":"2025-09-09T10:48:14","slug":"formalin-buffered-10-in-phosphate-buffer","status":"publish","type":"product","link":"https:\/\/delta.screenup.com\/en\/produit\/formalin-buffered-10-in-phosphate-buffer\/","title":{"rendered":"Formalin Buffered 10% in Phosphate Buffer"},"content":{"rendered":"<p data-start=\"118\" data-end=\"251\">Le <strong data-start=\"121\" data-end=\"153\">formol tamponn\u00e9 neutre (NBF)<\/strong> est un fixateur largement utilis\u00e9 en histologie, exclusivement pour la <strong data-start=\"225\" data-end=\"248\">microscopie optique<\/strong>.<\/p>\n<p data-start=\"253\" data-end=\"507\"><strong data-start=\"253\" data-end=\"270\">Composition :<\/strong><br data-start=\"270\" data-end=\"273\" \/>Le NBF est compos\u00e9 de <strong data-start=\"295\" data-end=\"311\">formald\u00e9hyde<\/strong> (g\u00e9n\u00e9ralement \u00e0 10 %, soit environ 4 % de formald\u00e9hyde en volume) dilu\u00e9 dans un <strong data-start=\"392\" data-end=\"412\">tampon phosphate<\/strong> pour obtenir un pH neutre. Le tampon phosphate assure la <strong data-start=\"470\" data-end=\"489\">stabilit\u00e9 du pH<\/strong> de la solution.<\/p>\n<p data-start=\"509\" data-end=\"529\"><strong data-start=\"509\" data-end=\"527\">Applications :<\/strong><\/p>\n<p data-start=\"531\" data-end=\"782\">Le NBF est utilis\u00e9 pour <strong data-start=\"555\" data-end=\"595\">pr\u00e9server les \u00e9chantillons de tissus<\/strong>, garantissant que leur structure cellulaire reste proche de l\u2019\u00e9tat in vivo. Il est particuli\u00e8rement recommand\u00e9 pour la pr\u00e9paration d\u2019\u00e9chantillons destin\u00e9s \u00e0 la <strong data-start=\"756\" data-end=\"779\">microscopie optique<\/strong>.<\/p>\n<ul data-start=\"784\" data-end=\"1580\">\n<li data-start=\"784\" data-end=\"1055\">\n<p data-start=\"786\" data-end=\"1055\"><strong data-start=\"786\" data-end=\"800\">Fixation :<\/strong> Lors de l\u2019immersion des tissus dans le NBF, le formald\u00e9hyde r\u00e9agit avec les prot\u00e9ines, formant une structure g\u00e9lifi\u00e9e qui conserve les composants cellulaires. Les tissus correctement fix\u00e9s supportent les \u00e9tapes suivantes de traitement et de coloration.<\/p>\n<\/li>\n<li data-start=\"1056\" data-end=\"1170\">\n<p data-start=\"1058\" data-end=\"1170\"><strong data-start=\"1058\" data-end=\"1074\">Histologie :<\/strong> Fixateur g\u00e9n\u00e9raliste de choix pour la pr\u00e9paration des tissus en vue d\u2019examens microscopiques.<\/p>\n<\/li>\n<li data-start=\"1171\" data-end=\"1416\">\n<p data-start=\"1173\" data-end=\"1416\"><strong data-start=\"1173\" data-end=\"1203\">Techniques de coloration :<\/strong> Utilis\u00e9 dans diverses techniques, notamment la coloration <strong data-start=\"1262\" data-end=\"1275\">Oil-Red-O<\/strong> pour diff\u00e9rencier les lign\u00e9es cellulaires pr\u00e9adipocytes et analyser des tissus tels que les yeux de lapin ou l\u2019intestin de rat diab\u00e9tique.<\/p>\n<\/li>\n<li data-start=\"1417\" data-end=\"1580\">\n<p data-start=\"1419\" data-end=\"1580\"><strong data-start=\"1419\" data-end=\"1433\">Stockage :<\/strong> Adapt\u00e9 \u00e0 la <strong data-start=\"1446\" data-end=\"1471\">fixation courte dur\u00e9e<\/strong> (id\u00e9al pour les laboratoires \u00e0 haut d\u00e9bit) ainsi qu\u2019au <strong data-start=\"1527\" data-end=\"1550\">stockage long terme<\/strong> des \u00e9chantillons de tissus.<\/p>\n<\/li>\n<\/ul>\n<p data-start=\"1582\" data-end=\"1791\"><strong data-start=\"1582\" data-end=\"1598\">Pr\u00e9caution :<\/strong><br data-start=\"1598\" data-end=\"1601\" \/>Le NBF est \u00e9tiquet\u00e9 pour <strong data-start=\"1626\" data-end=\"1682\">toxicit\u00e9, sensibilisation cutan\u00e9e et canc\u00e9rog\u00e9nicit\u00e9<\/strong>. Il est essentiel de prendre toutes les pr\u00e9cautions n\u00e9cessaires lors de la manipulation de cette solution.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2022\/09\/FDS-formaldehyde-neutre-10-dans-tampon-phosphate-version1-F.pdf\">MSDS version FR<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p data-start=\"42\" data-end=\"121\"><strong data-start=\"42\" data-end=\"119\">Tamponn\u00e9 au phosphate \u2013 uniquement pour histologie et microscopie optique<\/strong><\/p>\n<ul>\n<li data-start=\"125\" data-end=\"187\">Ne pas utiliser pour la fixation en microscopie \u00e9lectronique<\/li>\n<li data-start=\"125\" data-end=\"187\">pH : 6,9 \u00e0 7,1<\/li>\n<li data-start=\"125\" data-end=\"187\">Eau distill\u00e9e, tampon phosphate<\/li>\n<li data-start=\"125\" data-end=\"187\">\u00c0 conserver \u00e0 temp\u00e9rature ambiante (TA)<\/li>\n<li data-start=\"125\" data-end=\"187\">Fabriqu\u00e9 en France<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2022\/09\/FDS-formaldehyde-neutre-10-dans-tampon-phosphate-version1-F.pdf\">MSDS version FR<\/a><\/p>\n","protected":false},"featured_media":21261,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"none","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_nectar_blocks_hide_post_title":false,"_nectar_blocks_transparent_header_effect":false,"_nectar_blocks_transparent_header_effect_color":"light","_nectar_blocks_header_animation":false,"_nectar_blocks_header_animation_delay":0,"_nectar_blocks_header_animation_effect":"fade","_nectar_blocks_page_css":"","_nectar_blocks_page_js":""},"product_brand":[1361],"product_cat":[1362,1349,1366,1350],"product_tag":[],"class_list":["post-21260","product","type-product","status-publish","has-post-thumbnail","product_brand-em-grade-com","product_cat-fixateurs","product_cat-met","product_cat-paraformaldehydes","product_cat-reactifs","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"_links":{"self":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product\/21260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media\/21261"}],"wp:attachment":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media?parent=21260"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_brand?post=21260"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_cat?post=21260"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_tag?post=21260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}