Millonig (1964); Karlsson and Schultz (1965)<\/p>\n
Ingredients:<\/strong> Sodium Phosphate, monobasic (NaH2PO4xH2O) Sodium Phosphate, dibasic (Na2HPO4xH2O) 0.5% Sodium Chloride (NaCl)<\/p>\n Shipping Conditions: Ambient Temperature\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0The pH of this buffer is 7.4 (0.1M);<\/p>\n Millonig\u2019s prepare<\/strong> the osmolarity is 440 mosmols<\/strong>, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use. Storage : 0-5\u00b0C MSDS_11582<\/a> \u00a0<\/p>\n","protected":false},"excerpt":{"rendered":" Millonig (1964); Karlsson and Schultz (1965)<\/p>\n Ingredients:<\/strong> Sodium Phosphate, monobasic (NaH2PO4xH2O) Sodium Phosphate, dibasic (Na2HPO4xH2O) 0.5% Sodium Chloride (NaCl) Shipping Conditions: Ambient Temperature The pH of this buffer is 7.4 (0.1M);<\/p>\n the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids.<\/p>\n \u00a0<\/p>\n Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms<\/strong>, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.<\/p>\n
1. Phosphate buffer (1961)
Sol A:<\/strong> 2.26% monobasic sodium phosphate in water
Sol B<\/strong>: 2.52% NaOH in water
Buffer final concentration: 0.13 M<\/strong>
Mix 41.5 ml of Sol A with 8.5 ml Sol B
Remove 5 ml of mix and add 5 ml of 4% sucrose solution.
Add 25g of MgCl2 or CaCl2 to each 100 ml of buffer
Adjust pH to 7.3 with Soln B
Stable for several weeks at 4 C
2. Phosphate buffer (1964)
In 500 ml dH2O:
Monobasic sodium phosphate 1.8 g
Dibasic sodium phosphate 23.25 g
NaCl 5 g
Ph to desired value then add
d H2O to make 1,000 ml<\/p>\n