{"id":35799,"date":"2026-08-27T11:55:47","date_gmt":"2026-08-27T09:55:47","guid":{"rendered":"https:\/\/delta.screenup.com\/produit\/phosphate-buffer-0-1m-2\/"},"modified":"2026-08-27T12:25:52","modified_gmt":"2026-08-27T10:25:52","slug":"phosphate-buffer-0-1m-2","status":"publish","type":"product","link":"https:\/\/delta.screenup.com\/en\/produit\/phosphate-buffer-0-1m-2\/","title":{"rendered":"Phosphate Buffer, 0.1M*"},"content":{"rendered":"\n

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only *Meant for biological research only. Not to be used for medical or any other purposes. \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 MSDS_19340-60<\/a> \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0MSDS_19340-65<\/a> \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0MSDS_19340-70<\/a> \u00a0\u00a0 \u00a0\u00a0 \u00a0\u00a0 \u00a0MSDS_19340-72<\/a> \u00a0 \u00a0<\/p>

info+<\/strong><\/span><\/p>

PB (0.1 M phosphate buffer pH 7.2)<\/span><\/strong><\/h2>
MATERIALS<\/span><\/strong><\/div><\/div>

Reagents<\/span><\/strong><\/h4>

Sodium phosphate dibasic<\/span><\/p>

Sodium phosphate monobasic (anhydrous)<\/span><\/p><\/div>

Equipment<\/span><\/strong><\/h4>

Beakers<\/span><\/p>

Graduated cylinders<\/span><\/p>

Hot plate (optional; see Step 1 note)<\/span><\/p>

Scale<\/span><\/p>

Spatulas<\/span><\/p>

Stir plate<\/span><\/p>

Weigh boats<\/span><\/p><\/div><\/div>

\u00a0<\/div><\/div>

METHOD<\/strong><\/span><\/h3>

  • 1.\u00a0<\/span>Prepare sodium phosphate dibasic stock (0.5 M) by dissolving 35.5 g of sodium phosphate dibasic in a final volume of 500 mL of H2<\/sub>O.<\/span><\/p>

    Some crystallization will occur when the solution is stored at 4\u00baC. Warm on a hot plate and stir until the crystals dissolve.<\/em><\/span><\/p><\/li>

  • 2.\u00a0<\/span>Prepare sodium phosphate monobasic stock (0.5 M) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of 500 mL of H2<\/sub>O.<\/span><\/p><\/li>

  • 3.\u00a0<\/span>Prepare 0.1 M sodium phosphate dibasic: Put 80 mL of sodium phosphate dibasic stock (0.5 M) from Step 1 in a beaker and add H2<\/sub>O to give a final volume of 400 mL.<\/span><\/p><\/li>

  • 4.\u00a0<\/span>Prepare 0.1 M sodium phosphate monobasic: Put 30 mL of sodium phosphate monobasic stock (0.5 M) from Step 2 in a beaker and add H2<\/sub>O to give a final volume of 150 mL.<\/span><\/p><\/li>

  • 5.\u00a0<\/span>Bring the 0.1 M sodium phosphate dibasic solution from Step 3 to pH 7.2 by adding as much as needed of the 0.1 M sodium phosphate monobasic solution from Step 4.<\/span><\/p>

    The resulting solution is 0.1 M phosphate buffer pH 7.2.<\/em><\/span><\/p><\/li><\/ul><\/div>\n","protected":false},"excerpt":{"rendered":"

    Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only *Meant for biological research only. Not to be used for medical or any…<\/p>\n","protected":false},"featured_media":21236,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_nectar_blocks_hide_post_title":false,"_nectar_blocks_transparent_header_effect":false,"_nectar_blocks_transparent_header_effect_color":"light","_nectar_blocks_header_animation":false,"_nectar_blocks_header_animation_delay":0,"_nectar_blocks_header_animation_effect":"fade","_nectar_blocks_page_css":"","_nectar_blocks_page_js":""},"product_brand":[],"product_cat":[1349,1364,1350,1353],"product_tag":[],"class_list":["post-35799","product","type-product","status-publish","has-post-thumbnail","product_cat-met","product_cat-phosphate","product_cat-reactifs","product_cat-tampons","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"acf":[],"_links":{"self":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product\/35799","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media\/21236"}],"wp:attachment":[{"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/media?parent=35799"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_brand?post=35799"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_cat?post=35799"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/delta.screenup.com\/en\/wp-json\/wp\/v2\/product_tag?post=35799"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}