Fluorescence Adapter for Hirox Digital Microscope

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The NIGHTSEA Model SFA Fluorescence Adapter system can add a versatile fluorescence imaging capability to the Hirox digital microscope. Visit our gallery of images made with the NIGHTSEA system and the Hirox microscope.

Description

Ordering Information_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Best Value Full System KitIncludes three wavelengths (UV + VI + RB)
Single wavelength full systemsAvailable in non-UV or UV wavelengths
Add-on single wavelengths Available in non-UV or UV wavelengths
_x000D_  _x000D_ _x000D_ Fluorescence solutions _x000D_ _x000D_ The key elements of any fluorescence system are:_x000D_ – a light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest;_x000D_ – a barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample._x000D_ _x000D_ NIGHTSEA implements these for the Hirox microscope with:_x000D_ – high intensity LED light sources available in five excitation wavelength ranges (see list further down page);_x000D_ – emission barrier filters that can be added to the Keyence lenses easily and non-invasively._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Hirox microscope with NIGHTSEA fluorescence adapter  Detail of NIGHTSEA light head and barrier filter with Hirox microscope
_x000D_  _x000D_ _x000D_ Here is a sample of what you can see. For many more examples visit our Hirox image gallery._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Epoxy on motor shaft, 20x, UV excitationConcrete thin section, 20x, white light and fluorescence under Royal Blue excitationConformal coating on circuit board, 20x, fluorescence under UV excitation and white light lightBone in sand, 20x, fluorescence under UV excitation and white lightFluorescent mineral, 20x, white light and fluorescence under Royal Blue excitation
_x000D_  _x000D_ _x000D_ Comprehensive solution kit_x000D_ We have configured a versatile Hirox fluorescence adapter kit (part number SFA-K-IND) that includes the most useful excitation wavelengths for varied industry applications. The SFA-H-IND kit includes the following items, at a package price that is significantly less than purchasing the items separately:_x000D_ _x000D_ – Three excitation light heads – Ultraviolet (UV), Violet (VI), and Royal Blue (RB) . This recommendation is based on our experience with varied applications. Read the explanation below._x000D_ – Three barrier filters paired to the excitation light heads_x000D_ – Matching barrier filter glasses for each excitation wavelength_x000D_ – Gooseneck lamp base with dimming control_x000D_ – Universal power supply – 120/240VAC, 50/60Hz, with international plug set_x000D_ – Packing/carrying case with custom-cut foam_x000D_ _x000D_ We do offer additional wavelength sets that may be of use for other applications._x000D_ _x000D_ Why we recommend a set of three excitation wavelengths for industry needs_x000D_ _x000D_ We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:_x000D_ _x000D_ – A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence._x000D_ _x000D_ – Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence._x000D_ _x000D_ – A prospective customer sent us a sample of material that was known to fluoresce under UV. The goal was to see the distribution of non-fluorescent particles in a fluorescent matrix. While both UV and Royal Blue excited fluorescence in the sample, the best imaging contrast was achieved with Violet excitation._x000D_ _x000D_ Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise._x000D_ _x000D_ Wavelength sets_x000D_ _x000D_ There are five excitation/emission wavelength combinations available for the system._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
ColorDesignationExcitationEmission
UltravioletUV360 – 380nm415nm longpass
VioletVI400 – 415nm450nm longpass
Royal BlueRB440 – 460nm500nm longpass
CyanCY490 – 515nm550nm longpass
GreenGR510 – 540nm600nm longpass
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