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Verhoeff’s Van-Gieson’s (VVG Method)

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 This method is used for identifying elastic fibers in tissues such as skin, aorta, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The elastic fibers will be stained blue-black and background will be stained yellow.

Sheehan and Hrapchak (1980)
For stain elastic fibers, nuclei, collagen and other tissue elements.

Verhoffs Van Geison protocol

MSDS 26374-01 MSDS 26374-02 MSDS 26374-03 MSDS 26374-04 MSDS 26374-05 MSDS 26374-06

 

Description

Description: This method is used for identifying elastic fibers in tissues such as skin, aorta, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The elastic fibers will be stained blue-black and background will be stained yellow.

Stain results:
Elastic Fibers: Blue-black to Black – Fine elastic fibrils
may not be stained with this method
Nuclei: Blue to Black
Collagen: Red
Other Tissue Elements: Yellow

References:
Sheehan, Dezna C., Hrapchack, Barbara B.: Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby C., St Louis, p. 196-197, c. 1980