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Fluorescence Adapter for Keyence VHX Series Digital Microscope

1,00 €

The NIGHTSEA Model SFA Fluorescence Adapter system adds a versatile fluorescence imaging capability to the Keyence VHX series of digital microscopes. The system is being used successfully by a growing number of Keyence owners for a variety of applications. Visit our gallery of images made with the NIGHTSEA adapter and the Keyence microscope.

UGS : SFA-KZ Catégories : , , , Marque :

Description

Ordering Information_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
SFA-KZ2-INDBest Value Full System Kit_x000D_ Includes three wavelengths (UV + VI + RB), supports ZST, Z100, Z50, Z20, Z00
SFA-KZST-INDZST Full System Kit_x000D_ Includes three wavelengths (UV + VI + RB), supports ZST and Z100
SFA-KZB-INDBasic Full System Kit_x000D_ Includes three wavelengths (UV + VI + RB), supports Z00, Z20, Z50 only
SFA-KZ100-INDZ100 Full System Kit_x000D_ Includes three wavelengths (UV + VI + RB), supports Z100 only
SFA-KZ1B-INDZ1B Full System Kit_x000D_ Includes three wavelengths (UV + VI + RB), supports Z00, Z20, Z50, and Z100_x000D_ (SFA-KZB-IND and SFA-KZ100-IND combined)
_x000D_  _x000D_ _x000D_ Fluorescence solutions for most Keyence lenses_x000D_ _x000D_ Keyence offers a variety of lenses for imaging at different scales. NIGHTSEA offers several variants of our fluorescence adapter system that work with the most commonly used Keyence lenses – the ZST, Z100, Z50, Z20, and Z00._x000D_ The key elements of any fluorescence system are:_x000D_ – a light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest;_x000D_ – a barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample._x000D_ _x000D_ NIGHTSEA implements these for the Keyence system with:_x000D_ – high intensity LED light sources available in five excitation wavelength ranges (see list further down page);_x000D_ – emission barrier filters that can be added to the Keyence lenses easily and non-invasively._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Fluorescent mineral sample imaged with the Keyence + NIGHTSEA systems Z00, Z20, and Z50 lenses – the barrier filter slips over the bottom of the lensZ100 and ZST lenses – the barrier filter inserts in the Analyzer slot at the top of the lens Keyence VH-ZST lens, fiber optic port with NIGHTSEA adapter and light head NIGHTSEA Light Head Mount for Keyence VH-ZST lens NIGHTSEA focus spot with Keyence VH-ZST lens at high magnification
_x000D_  _x000D_ _x000D_ Here is a sample of what you can see. For many more examples visit our Keyence image gallery._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Cement thin section fluorescence, VH-Z100 lens, Royal Blue excitation, 700xNylon granule with gel defect, VH-ZST lens, Royal Blue excitation, 100xElectronic component failure analysis, VH-ZST lens, Royal Blue excitation, 500xEpoxy on motor shaft, VH-ZST lens, Ultraviolet excitation, 20x
_x000D_  _x000D_ _x000D_ Comprehensive solution kit_x000D_ We have configured a versatile Keyence fluorescence adapter kit (part number SFA-K-IND) that includes the most useful excitation wavelengths for varied industry applications, and that adapts to all of the supported Keyence lenses. The SFA-K-IND kit includes the following items, at a package price that is significantly less than purchasing the items separately:_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
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Custom case for 3-color system for Keyence
_x000D_ – Three excitation light heads – Ultraviolet (UV), Violet (VI), and Royal Blue (RB) . This recommendation is based on our experience with varied applications. Read the explanation below._x000D_ – Two sets of three barrier filters – one set for the ZST and Z100 lenses, one set for the Z00, Z20, and Z50_x000D_ – Matching barrier filter glasses for each excitation wavelength_x000D_ – Accessory hardware for mounting the NIGHTSEA light head over the ZST fiber optic input port (enhanced performance at high magnification)_x000D_ – Gooseneck lamp base with dimming control_x000D_ – Universal power supply – 120/240VAC, 50/60Hz, with international plug set_x000D_ – Packing/carrying case with custom-cut foam_x000D_ _x000D_ We do offer additional wavelength sets that may be of use for other applications._x000D_ _x000D_ Why we recommend a set of three excitation wavelengths for industry needs_x000D_ _x000D_ We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:_x000D_ _x000D_ – A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence._x000D_ _x000D_ – Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence._x000D_ _x000D_ – A prospective customer sent us a sample of material that was known to fluoresce under UV. The goal was to see the distribution of non-fluorescent particles in a fluorescent matrix. While both UV and Royal Blue excited fluorescence in the sample, the best imaging contrast was achieved with Violet excitation._x000D_ _x000D_ – We achieve enhanced performance with the ZST lens by mounting the light head on the lens’s fiber optic port, but that path does not transmit UV well. In many cases, for example to inspect conformal coating, Violet is an excellent alternative to UV._x000D_ Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact us If you would like to discuss your application further._x000D_ _x000D_ Performance expectations_x000D_ _x000D_ The microscopes in the Keyence VHX series were not designed for fluorescence. That said, the NIGHTSEA adapter system adds a fluorescence capability that is proving to be valuable for a growing number of users. Several companies have purchased multiple adapters so that they could replicate the performance across multiple microscopes. In at least one case the customer only purchased their Keyence system because of the added NIGHTSEA fluorescence capability that enabled a critical analysis function for them._x000D_ Read more about performance expectations with the NIGHTSEA adapters on the Keyence microscopes._x000D_ _x000D_ Wavelength sets_x000D_ _x000D_ There are five excitation/emission wavelength combinations available for the system._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
ColorDesignationExcitationEmission
UltravioletUV360 – 380nm415nm longpass
VioletVI400 – 415nm450nm longpass
Royal BlueRB440 – 460nm500nm longpass
CyanCY490 – 515nm550nm longpass
GreenGR510 – 540nm600nm longpass
_x000D_ Enhanced performance with the VH-ZST_x000D_ _x000D_ With the ZST lens and the higher magnification (200 – 2000x) objective you can achieve enhanced performance by removing the Keyence fiber optic illuminator and positioning the NIGHTSEA excitation light head in its place with our custom adapter. This brings the excitation energy through the lens, creating a much more concentrated illumination spot than can be achieved with the light source positioned to the side. We have made fluorescence images at up to 2000x with this configuration._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Focused fluorescence excitation spot with VH-ZST high magnification objectiveFluorescent mineral (willemite) at 20 and 1000x, VH-ZST lens, Royal Blue excitationElectronic component failure analysis, VH-ZST lens, Royal Blue excitation, 2000x
_x000D_  _x000D_ _x000D_ Read more about achieving the best fluorescence results with the ZST lens.

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